Bovine Pulp Tissue Dissolution Ability of Irrigants Associated or Not to Ultrasonic Agitation.

The aim of this study was to evaluate the tissue dissolution ability of different irrigating solutions associated or not to ultrasonic agitation. Ninety bovine pulp fragments (n=10 per group) were weighed and then placed individually in Eppendorf test tubes containing the following irrigants: G1- 2.5% sodium hypochlorite (NaOCl); G2- 2.5% NaOCl + ultrasonic agitation (US); G3- 2.5% NaOCl + 0.2% cetrimide (CTR); G4- 2.5% NaOCl + 0.2% CTR + US; G5- 400 ppm Sterilox (SX); G6- SX + US; G7- 0.2% CTR; G8- 0.2% CTR + US; G9- saline solution. Two blinded observers assessed the samples continuously for the first 4 h, and then every hour for the next 12 h. Dissolution speed was calculated by dividing the initial pulp weight (mg) by the period of time until complete dissolution (min). Data were compared by ANOVA and Tukey post hoc test with a 5% significance level. G1 to G4 dissolved pulp fragments completely and G2 was significantly faster than the other groups. G5 to G9 did not exhibit dissolving activity. In conclusion, only groups containing NaOCl were capable of pulp tissue dissolution, which was enhanced by ultrasonic agitation, but did not alter when 0.2% cetrimide was associated. This isolated solution and Sterilox showed no dissolving capacity, regardless the use of ultrasound.

Bovine Pulp Tissue Dissolution Ability of Irrigants Associated or Not to Ultrasonic Agitation

Abstract: The aim of this study was to evaluate the tissue dissolution ability of different irrigating solutions associated or not to ultrasonic agitation. Ninety bovine pulp fragments (n=10 per group) were weighed and then placed individually in Eppendorf test tubes containing the following irrigants: G1- 2.5% sodium hypochlorite (NaOCl); G2- 2.5% NaOCl + ultrasonic agitation (US); G3- 2.5% NaOCl + 0.2% cetrimide (CTR); G4- 2.5% NaOCl + 0.2% CTR + US; G5- 400 ppm Sterilox (SX); G6- SX + US; G7- 0.2% CTR; G8- 0.2% CTR + US; G9- saline solution. Two blinded observers assessed the samples continuously for the first 4 h, and then every hour for the next 12 h. Dissolution speed was calculated by dividing the initial pulp weight (mg) by the period of time until complete dissolution (min). Data were compared by ANOVA and Tukey post hoc test with a 5% significance level. G1 to G4 dissolved pulp fragments completely and G2 was significantly faster than the other groups. G5 to G9 did not exhibit dissolving activity. In conclusion, only groups containing NaOCl were capable of pulp tissue dissolution, which was enhanced by ultrasonic agitation, but did not alter when 0.2% cetrimide was associated. This isolated solution and Sterilox showed no dissolving capacity, regardless the use of ultrasound.

Resumo: O objetivo deste estudo foi avaliar a capacidade de dissolução tecidual de diferentes soluções irrigadoras associadas ou não à agitação ultrassônica. Noventa fragmentos de polpa bovina (n=10 por grupo) foram pesados e em seguida colocados individualmente em tubos tipo Eppendorf contendo os seguintes irrigantes: G1- hipoclorito de sódio (NaOCl) a 2,5%; G2- NaOCl a 2,5% + agitação ultrassônica (US); G3- NaOCl a 2,5% + cetramida (CTR) a 0,2%; G4- NaOCl a 2,5% + CTR a 0,2% + US; G5- 400 ppm Sterilox (SX); G6- SX + US; G7- CTR a 0,2%; G8- CTR a 0,2% + US; G9- solução salina. Dois observadores cegados em relação aos grupos experimentais avaliaram as amostras continuamente durante as primeiras 4 h e depois a cada hora pelas próximas 12 h. A velocidade de dissolução foi calculada dividindo o peso inicial da polpa (mg) pelo período de tempo até sua dissolução completa (min). Os dados foram comparados por ANOVA e teste post hoc de Tukey com nível de significância de 5%. Os grupos G1 a G4 dissolveram os fragmentos de polpa completamente e G2 foi significativamente mais rápido do que os outros. Os grupos G5 a G9 não apresentaram atividade de dissolução. Em conclusão, apenas grupos contendo NaOCl foram capazes de dissolver tecido pulpar, o que foi melhorado pela agitação ultrassônica, mas não alterado quando CTR a 0,2% foi associada. Esta solução isolada e o Sterilox não mostraram nenhuma capacidade de dissolução, independentemente da utilização do ultrassom.